Useful Links

Training

The UTHealth Houston CryoEM facility encourages users to gain foundational knowledge essential for successful CryoEM studies. Customizable hands-on training that covers the full workflow, including instrument operation and data collection on both the Titan Krios G3 and TFS Glacios F30 microscopes is available to experienced users. For details on how to begin the training process, please contact Venkata Mallampalli or Mehdi Zarei.

Here is a downloadable PDF file containing a curated set of general introduction and online support resources related to CryoEM. These include conceptual overviews as well as links to external websites offering helpful materials. Please note that while these resources offer useful background, they may not reflect the specific instruments or configurations used at our facility. For guidance on software and tools specific to our setup, please reach out to the core staff or refer to the equipment descriptions on our iLab site.

Publications

Gating machinery of InsP ₃ R channels revealed by electron cryomicroscopy. Fan G, Baker ML, Wang Z, Baker MR, Sinyagovskiy PA, Chiu W, Ludtke SJ, Serysheva II. Nature. 2015 Oct 12.
Baker, M.R., Fan, G., Seryshev, A.B., Agosto M.A., Baker M.L., Serysheva I.I. Cryo-EM structure of type 1 IP₃R channel in a lipid bilayer. Commun Biol 4, 625 (2021).
Dosey TL, Wang Z, Fan G, Zhang Z, Serysheva II, Chiu W, Wensel TG. Structures of TRPV2 in distinct conformations provide insight into role of the pore turret. Nat Struct Mol Biol. 2019.
Li H, Huang CY, Govorunova EG, Schafer CT, Sineshchekov OA, Wang M, Zheng L, Spudich JL. Crystal structure of a natural light-gated anion channelrhodopsin. Elife. 2019.
Fan G., Baker M. R., Wang Z., Seryshev A. B., Ludtke S. J., Matthew L. Baker M. L., Serysheva I. I. Cryo-EM Reveals Ligand Induced Allostery Underlying InsP3R Channel Gating. Cell Research (Nature Publishing Group) 28: 1158-1170 (2018).
Fischer TD, Dash PK, Liu J, Waxham MN. Morphology of mitochondria in spatially restricted axons revealed by cryo-electron tomography. PLoS Biol. 2018.
Park D, Lara-Tejero M, Waxham MN, Li W, Hu B, Galán JE, Liu J.. Visualization of the type III secretion mediated Salmonella-host cell interface using cryo-electron tomography. Elife. 2018.
Baker MR, Fan G, Serysheva II. Structure of IP3R channel: high-resolution insights from cryo-EM. Curr Opin Struct Biol. 2017.
Wang Z, Fan G, Hryc CF, Blaza JN, Serysheva II, Schmid MF, Chiu W, Luisi BF, Du D. An allosteric transport mechanism for the AcrAB-TolC multidrug efflux pump. Elife. 2017.
Fan, G., Baker, M. L., Wang, Z., Baker, M. R., Sinyagovskiy, P. A., Chiu, W., Ludtke, S. J. & Serysheva, I. I.. Gating Machinery of IP3R Channels Revealed by Cryo-EM. Nature 2015.
Baker MR, Fan G, Serysheva II. Single-particle cryo-EM of the ryanodine receptor channel in an aqueous environment. Eur J Transl Myology. 2015.