Atomic Force Microscopy Core - Internal Medicine - NBME - UTHealth
Atomic Force Microscopy Core - Internal Medicine
Atomic Force Microscopy (AFM) is a technique for characterizing surfaces and can reproduce topological images under physiological-like conditions. AFM is a noninvasive technique base on bio-sensing. Scanning can be performed in air or in a liquid environment, and a range of samples, from living cells down to single molecules can be imaged. AFM is also an attractive tool for studying the endocytosis of nanovectors and the systemic response to biological processes. An area of major interest is to determine the stiffness of a sample (Elastic Modulus). The elasticity of the cell membrane can vary between cell types as a function of growth, differentiation, disease or treatment. Non-cellular structures can also be imaged.
SERVICE CAPABILITIES OF THE AFM FACILITY
- Combined AFM and fluorescence/bright-field imaging
- Topographical Imaging
- High-Resolution Imaging
- Time-lapse Imaging - monitor sample morphology over time
- Nano-probing - measure forces between molecules
- Measure elasticity
- Measure stiffness
- Measure roughness
The AFM Core uses a BioscopeTM II atomic force microscope (Bruker Corp., Santa Barbara, CA) that requires minimal sample preparation. The BioscopeTM II is integrated with a Nikon TE2000 inverted optical microscope to simultaneously acquire bright-field and fluorescence images of samples under study.
The Atomic Force Microscopy Core Facility is situated in room 3SCR6.3728 of the South Campus Research Building Number 3, located at 1881 East Road, Houston, 77054.
For more information or to schedule a meeting, please contact:
- Dr. Ana Maria Zaske, Manager
- Dr. David Volk, Director
- J. Scott Harrison, Business Mgr.
ATOMIC FORCE MICROSCOPY CORE FACILITY PRICE SHEET*
Internal Medicine – Atomic Force Microscopy Core Facility
ATOMIC FORCE IMAGING
Developmental Hourly Rate
Standard AFM Hourly Rate
*Overtime rates of 1.5 times the normal rates apply to all projects requiring over 8 hours/day
$ 1.5x fee
$ 1.5x fee
UTHealth provides a variety of service centers and core facilities to provide expert guidance and services to the UTHealth and wider Texas Medical Center community.
UTHEALTH SERVICE CENTERS AND CORE FACILITIES
SERVICE CENTRER LINKS
SERVICE CENTER DESCRIPTIONS
AFM and fluorescence imaging of tissue. Contact Dr. Ana-Maria Zaske for more information.
Flow Cytometry detection and separation with detection of up to eight colors simultaneously. Contact Director Eva Sevick or Manager Dr. Amy Hazen for more information.
Histopathology Services and Dissections. Contact Director Eva Sevick or Manager Sarah Amra for more information.
Molecular diagnostic testing in a CLIA certified laboratory. MS-based clinical metabolites and Vitamin D, and discovery phase studies. Contact Director Dr. Kevin Rosenblatt for more information.
Mass spectroscopy and affinity based proteomics services suitable for discovery or clinical research. Contact Dr. Kevin Rosenblatt for more information.
The AFM Core is financially supported by The Center for Clinical and Translational Sciences (NIH/NCATS UL1 TR000371) and the Department of Internal Medicine
This page was last modified by David.Volk@uth.tmc.edu on May 23, 2014.