The Department of NanoMedicine and BioMedical Engineering

Atomic Force Microscopy Core - Internal Medicine - NBME - UTHealth

Atomic Force Microscopy Core - Internal Medicine

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Atomic Force Microscopy (AFM)  is a technique for characterizing surfaces and can reproduce topological images under physiological-like conditions. AFM is a noninvasive technique base on bio-sensing. Scanning can be performed in air or in a liquid environment, and a range of samples, from living cells down to single molecules can be imaged. AFM is also an attractive tool for studying the endocytosis of nanovectors and the systemic response to biological processes. An area of major interest is to determine the stiffness of a sample (Elastic Modulus). The elasticity of the cell membrane can vary between cell types as a function of growth, differentiation, disease or treatment. Non-cellular structures can also be imaged. 

 

 

SERVICE CAPABILITIES OF THE AFM FACILITY

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  • Combined AFM and fluorescence/bright-field imaging
  • Topographical Imaging
  • High-Resolution Imaging
  • Time-lapse Imaging - monitor sample morphology over time
  • Nano-probing - measure forces between molecules
  • Measure elasticity
  • Measure stiffness
  • Measure roughness

EQUIPMENT

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The AFM Core uses a BioscopeTM II atomic force microscope (Bruker Corp., Santa Barbara, CA) that requires minimal sample preparation. The BioscopeTM II is integrated with a Nikon TE2000 inverted optical microscope to simultaneously acquire bright-field and fluorescence images of samples under study.

The Atomic Force Microscopy Core Facility is situated in room 3SCR6.3728 of the South Campus Research Building Number 3, located at 1881 East Road, Houston, 77054.

 

CONTACT INFORMATION

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For more information or to schedule a meeting, please contact:

  • Dr. Ana Maria Zaske, Manager
    Ana.M.Zaske@uth.tmc.edu
    (713) 486-5418
  • Dr. David Volk, Director
    David.Volk@uth.tmc.edu
    (713) 500-2232
  • J. Scott Harrison, Business Mgr.
    James.S.Harrison@uth.tmc.edu
    (713) 500-7968

     

    PRICING INFORMATION

    ATOMIC FORCE MICROSCOPY CORE FACILITY PRICE SHEET*

    Internal Medicine – Atomic Force Microscopy Core Facility

    Internal

    External

    ATOMIC FORCE IMAGING

     

     

    Developmental Hourly Rate

    $            50

    $        100

    Standard AFM Hourly Rate

    $            85

    $        150

    Specialty Probes

    $            100

    $        200

    POST-ACQUISITION ANALYSIS

     

     

    Elasticity Calculations

    $            40

    $        80

    Image Enhancements

    $            40

    $        80

    OVERTIME RATES*

     

     

    *Overtime rates of 1.5 times the normal rates apply to all projects requiring over 8 hours/day

    $            1.5x fee

    $        1.5x fee

     

     

    UTHealth provides a variety of service centers and core facilities to provide expert guidance and services to the UTHealth and wider Texas Medical Center community.

    UTHEALTH SERVICE CENTERS AND CORE FACILITIES

    SERVICE CENTRER LINKS

    SERVICE CENTER DESCRIPTIONS

    Atomic Force Microscopy Core

    AFM and fluorescence imaging of tissue. Contact Dr. Ana-Maria Zaske for more information.

    Bioinformatics Service Center

    Statistical analysis of genetics, proteomics and metabolomic (metabonomic) data. Contact Co-Directors Dr. Jeffrey T. Chang or Dr. David Volk for more information.

    Flow Cytometry

    Flow Cytometry detection and separation with detection of up to eight colors simultaneously. Contact Director Eva Sevick or Manager Dr. Amy Hazen for more information.

    Histopathology Service Center

    Histopathology Services and Dissections. Contact Director Eva Sevick or Manager Sarah Amra for more information.

    Microscopy Service Center

    Fluorescence and confocal microscopy and image analysis services. Contact Director Dr. Eva Zsigmond or Manager Dr. Zhengmei (Mei) Mao for more information.

    Molecular Diagnostics

    Molecular diagnostic testing in a CLIA certified laboratory. MS-based clinical metabolites and Vitamin D, and discovery phase studies. Contact Director Dr. Kevin Rosenblatt for more information.

    Nano Chemistry Service Center

    Aptamer development, synthesis, labeling, and conjugation. Particle characterization. Contact Director Dr. David Volk or Manager Dr. Varatharsa Thiviyanathan for more information.

    Clinical and Translational Proteomics

    Mass spectroscopy and affinity based proteomics services suitable for discovery or clinical research. Contact Dr. Kevin Rosenblatt for more information.

    Transgenic Mice and Stem Cells

    Production of transgenic /knockout mice, cryopreservation, and stem cell services. Contact Director Dr. Eva Zsigmond or Manager Aleksey Domozhirov for more information.

    The AFM Core is financially supported by The Center for Clinical and Translational Sciences (NIH/NCATS UL1 TR000371) and the Department of Internal Medicine

    This page was last modified by David.Volk@uth.tmc.edu on May 23, 2014.