Transgenic and Stem Cell Services
The Transgenic and Stem Cells Core Facility was established in 1998 and since that time, it has generated over 650 new transgenic and knock-out mouse animal models for investigators from UTHealth, as well as for scientists from numerous other academic institutions.
The stem cell lines that have been derived in the laboratory are highly effective for the generation of knock-out/ knock-in mice and for cell differentiation studies. In addition to the production, cryopreservation and re-derivation of genetically-engineered mice and rats, the services of the facility also include gene targeting, derivation of new cell lines and intellectual/technical support in different aspects of microsurgery, cell culture and stem cells research.
SUMMARY OF SERVICES FORMS (PDF forms available below)
- Cell Line Request
- Cryopreservation Service Request
- Transgenic Service Request
- Embryonic Stem Cell Service Request
- Re-Derivation Service Request
- Training Request
|
SUMMARY OF SERVICES |
FEES |
|
|
A. MICROINJECTION |
UTH Rates |
Non UT Rates |
|
Transgenic Mice |
$ 3,700 |
$ 4,070 |
|
Knock-out mice 129/SvIMJ ES cells (per day) |
$ 1,900 |
$ 2,090 |
|
Knock-out mice C57BL/6ES cells (per day) |
$ 2,200 |
$ 2,420 |
|
Knock-out mice BALB/c ES cells (per day) |
$ 2,200 |
$ 2,420 |
| B. CRYOPRESERVATION |
UT Rates |
Non UT Rates |
|
Fertilized eggs |
$ 600 |
$ 660 |
|
Sperm (per line, minimum of 3 lines) |
$ 1,000 |
$ 1,100 |
|
Annually Renewable Storage Fee (1st line) ($25/2nd line & up) |
$ 50 |
$ 55 |
| C. RE-DERIVATION |
UT Rates |
Non UT Rates |
|
Re-Derivation |
$ 1,050 |
$ 1,155 |
| D. ES CELL SERVICES |
UT Rates |
Non UT Rates |
|
Gene targeting and selection |
$ 7,500 |
$ 8,250 |
|
Expansion and cryopreservation |
$ 650 |
$ 715 |
|
Derivation of MEF feeder cells |
$ 2,000 |
$ 2,200 |
| E. AVAILABLE CELL LINES |
UT Rates |
Non UT Rates |
|
Mouse ES cells (1 vial, passage 11) 129 /SVIM J |
$ 600 |
$ 660 |
|
Mouse ES cells (1 vial, passage 11) C57/BL/6 or BALB/c |
$ 900 |
$ 990 |
|
MEF feeder cells (passage 3) 129/SvIMJ or C57BL6 |
$ 100 |
$ 110 |
|
MEF feeder cells (passage 3) DR-4 |
$ 100 |
$ 110 |
Contacts:
Eva M. Zsigmond, PhD, Director
Phone: 713.500.2453 | Email: Eva.M.Zsigmond@uth.tmc.edu
Aleksey Domozhirov, Research Associate / Manager
Phone: 713.500.2452 | Email: Aleksey.Y.Domozhirov@uth.tmc.edu
UTHEALTH
The Brown Foundation Institute Of Molecular Medicine
1825 Pressler Street SRB 637B
Houston, Texas 77030
