The Brown Foundation Institute of Molecular Medicine for the Prevention of Human Diseases

Flow Cytometry

BDFCSARIA II The Brown Foundation Institute for Molecular Medicine (IMM) Flow Cytometry Service Laboratory is located on the sixth floor of the Fayez S. Sarofim Research Building and maintains four instruments, a BD FACSAria II, a BD FACSCalibur, a Coulter FC500 and a Luminex LX200. These specialized instruments allow researchers to evaluate a large number of samples at the cellular level in a short time frame. The Flow Cytometry Service Unit is committed to the continuing development of biological research and provides training, instrumentation, technical expertise, data analysis and interpretation for many IBC approved studies.

Instrumentation

The Luminex system enables the multiplex of up to 100 analytes in a single microplate well, using very small sample volumes. The system delivers fast and cost-effective bioassay results.

The BD FACSCalibur provides user-friendly multicolor analysis capabilities. It is a six parameter instrument equipped with 488nm and 635nm lasers, capable of detecting up to four fluorescent signals.

The Coulter FC500 is capable of detecting five fluorescent signals and provides a 32 tube carousel for automated sample acquisition.

The BD FACSAriaII is a state-of-the-art 19 parameter instrument equipped with six fiber-optic-linked solid-state lasers, a 355nm UV laser, a 488nm Blue laser, a 561nm Yellow-Green laser, a 640nm Red laser, a 405nm Violet laser, and a highly specialized 785nm Near-InfraRed laser, capable of detecting up to 17 fluorescent signals. The AriaII can perform two and four way bulk sorting for a variety of tube sizes as well as automatic cell deposition for single cell sorting into multiwell plates or onto microscope slides.

Rates for UTHealth Investigators (Effective 1/15/13)

Luminex $50 /use
Calibur (up to 4 color analysis) $40 /hour
FC 500 (up to 5 color analysis) $50 /hour
Aria II (unassisted) $100 /hour
Aria II (assisted) $125 /hour

Services are also available to users outside of UTHealth. Please contact Amy Hazen for pricing.

Our Staff:

Eva M. Sevick, PhD | Professor & Director
Direct Line: 713-500-3560
Email: Eva.Sevick@uth.tmc.edu 

Amy Hazen, PhD | Flow Cytometry Research
Direct Line: 713-500-3612
Email: Amy.Hazen@uth.tmc.edu 

Alainna D. Talton |Administrative Assistant III
Direct Line: 713-500-3488
Email: Alainna.D.Talton@uth.tmc.edu

For scheduling or questions regarding Flow Cytometry Services please call Dr. Amy Hazen