Dr. Jack C. Waymire
The University of Texas Health Science Center at Houston
Department of Neurobiology and Anatomy
- Neuronal regulation of neurotransmitter differentiation, biochemistry and secretory function in catecholamine neurotransmitter secreting cells
The long-range objective of research in Dr. Waymire's laboratory is to determine the cellular and molecular changes required to establish catecholamine neurotransmitter secretion from adult and various stem cell lines. The goals of this work is to establish the underlying molecular and cellular processes responsible for the catecholamine phenotype and modulation of catecholamine biosynthesis and secretion within these cells. His laboratory accomplishes this studies to understand how tyrosine hydroxylase, the enzyme that regulates catecholamine biosynthesis, and several other catecholamine-specific proteins are regulated. His laboratory is currently working to understand: 1) the role of the phosphorylation 2) the role of the transcription and 3) the role of mRNA stabilization in modulating these catecholamine specific proteins.
A tutorial in Dr. Waymire's laboratory will provide experience in protein purification, HPLC peptide purification, protein phosphorylation, immunoblotting, mRNA analysis, in situ protein expression, mutagenesis, enzyme assays, and primary tissue culture.