Dr. David L. Mitchell
The University of Texas MD Anderson Cancer Center
Department of Molecular Carcinogenesis
The Virginia Harris Cockrell Cancer Research Center
My laboratory is located in Smithville, Texas, at UT Science Park
DNA damage and repair. Current studies include investigating how DNA damaged by ultraviolet light (UVL) might affect gene expression indirectly through its effects on regulatory pathways. In this regard, we are focusing on two DNA-protein interactions that influence gene expression; 1) transcription factor binding to damaged promoters and 2) nucleosome reassembly on a damaged template. In addition, we are characterizing and purifying a novel human DNA damage binding protein.
Photocarcinogenesis. Initiation and promotion of skin tumors in mice irradiated with solar UV radiation is currently under study. Several aspects of this problem are being investigated, including 1) the use of immunological assays to compare the abilities of UVA and UVB-specific photodamage to initiate and promote carcinogenesis, 2) determine the effects of diet (e.g., saturated and unsaturated fats, green tea) on photocarcinogenesis, and 3) elucidate the role of the p53 tumor suppressor gene in nucleotide excision repair in mouse skin using transgenic mice.
Environmental genetic toxicology. We have developed sensitive immunological assays to measure DNA damage produced by various genotoxic agents in biological systems. DNA damages under study include cyclobutane pyrimidine dimers, (6-4) photoproducts, Dewar pyrimidinones, and monobasic oxidation damage produced by UVA and UVB solar radiation and benzo[a]pyrene diolepoxide damage in DNA. These analyses are being developed as biomonitors for assessing environmental pollution and as tools to evaluate the effects of anthropogenic disturbance (i.e., chemical pollution, deozonation) on natural ecosystems.
Program in Molecular Carcinogenesis
Office: MDA SPRD (Unit 116)
Ph.D. - The University of Texas-Austin - 1988